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Applications
Tetracyclines
10409
Separation Mode: Reversed Phase
Reversed Phase
RP-1
HPLC
Anti-Bacterial Agents
Pharmaceutical
Therapeutic / Clinical

Applications

Tetracyclines
Analytes
14-Epioxy tetracycline
2Oxytetracycline
3Tetracycline

Analyte not found

Details
LC Conditions (App ID: 10409)
Column:
Brand Name:
Part No:
Phase Name:
RP-1
Elution Type:
Isocratic
Mobile Phase:
A: 50g Tertiary butyl alcohol in water
B: Phosphate buffer, pH 7.5
C: Tetrabutyl ammonium hydrogen sulfate
D: 10mL EDTA disodium solution in water
Gradient Profile:
Step No.Time(min)%A%B%C%D
10206569
Flow Rate:
1 mL/min
Temperature:
60°C
Detection:
UV-Vis Abs.-Variable Wave.(UV) @ 260 nm (22°C)
Sample Note:
SecurityGuard™ Guard Cartridge System extends column lifetime. - SecurityGuard Cartridges, RP-1 4 x 3.0mm, 10/Pk Part No.: AJ0-5809 - Holder Part No.: KJ0-4282

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