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Applications
Corticosteroids using Ammonium Hydroxide
11073
Separation Mode: Reversed Phase
Reversed Phase
C18(2)
HPLC
Clinical
Pharmaceutical
Therapeutic / Clinical

Applications

Corticosteroids using Ammonium Hydroxide
Analytes
1Estriol
2Estradiol
3Estrone
Compound Name
Estriol
CID
5756
Molecular Formula
C18H24O3
Molecular Weight
288
No. Hydrogen Bond Acceptors
3
No. Hydrogen Bond Donors
3
Details
LC Conditions (App ID: 11073)
Column:
Brand Name:
Part No:
Phase Name:
C18(2)
Elution Type:
Gradient
Mobile Phase:
A: Water w/0.1% ammonium hydroxide, pH 10
B: Acetonitrile w/0.1% ammonium hydroxide, pH 10
Gradient Profile:
Step No.Time(min)%A%B
108515
232575
Flow Rate:
2 mL/min
Temperature:
25°C
Detection:
UV-Vis Abs.-Variable Wave.(UV) @ 205 nm (22°C)
Sample Note:
SecurityGuard™ Guard Cartridge System extends column lifetime. - SecurityGuard Cartridges, C18 4 x 3.0mm, 10/Pk Part No.: AJ0-4287 - Holder Part No.: KJ0-4282

Order Items Used in This Application

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