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Applications
Steroids
1198
Separation Mode: Reversed Phase
Reversed Phase
C18(2)
HPLC
Anti-Inflammatory Agents
Clinical
Pharmaceutical
Therapeutic / Clinical

Applications

Steroids
Analytes
1Hydrocortisone
2Corticosterone
311-alpha-Hydroxyprogesterone
4Cortisone acetate
511-Ketoprogesterone
Compound Name
Hydrocortisone
CID
5754
Molecular Formula
C21H30O5
Molecular Weight
362
No. Hydrogen Bond Acceptors
5
No. Hydrogen Bond Donors
3
Details
LC Conditions (App ID: 1198)
Column:
Brand Name:
Part No:
Phase Name:
C18(2)
Elution Type:
Isocratic
Mobile Phase:
A: 0.1 H3PO4
B: Acetonitrile
C: Methanol
Gradient Profile:
Step No.Time(min)%A%B%C
10543511
Flow Rate:
1 mL/min
Temperature:
22°C
Detection:
UV-Vis Abs.-Variable Wave.(UV) @ 254 nm (22°C)
Sample Note:
SecurityGuard™ Guard Cartridge System extends column lifetime. - SecurityGuard Cartridges, C18 4 x 3.0mm, 10/Pk Part No.: AJ0-4287 - Holder Part No.: KJ0-4282

Order Items Used in This Application

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