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Applications
Water-soluble vitamins standard on Synergi Hydro-RP
14234
Separation Mode: Reversed Phase
Reversed Phase
Hydro-RP
HPLC
Vitamin B Complex
Pharmaceutical
Therapeutic / Clinical

Applications

Water-soluble vitamins standard on Synergi Hydro-RP
Analytes
1Niacinamide
2Pantothenic acid
3p-Aminobenzoic acid
4Pyridoxine
5Thiamine
6Vitamin B12
7Riboflavin
Compound Name
Nicotinamide
CID
936
Molecular Formula
C6H6N2O
Molecular Weight
122
No. Hydrogen Bond Acceptors
2
No. Hydrogen Bond Donors
1
Details
LC Conditions (App ID: 14234)
Column:
Brand Name:
Part No:
Phase Name:
Hydro-RP
Elution Type:
Gradient
Mobile Phase:
A: 20mM KH2PO4 + 0.1% Hexane sulfonate, pH 3.0
B: Acetonitrile
Gradient Profile:
Step No.Time(min)%A%B
10973
23973
3185050
Flow Rate:
1.5 mL/min
Temperature:
22°C
Detection:
UV-Vis Abs.-Variable Wave.(UV) @ 210 nm (22°C)
Sample Note:
SecurityGuard™ Guard Cartridge System extends column lifetime. - SecurityGuard Cartridges, AQ C18 4 x 3.0mm, 10/Pk Part No.: AJ0-7511 - Holder Part No.: KJ0-4282

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