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Extraction of Bronchodilators on Strata™ X

Sample Preparation Details

Part No:8B-S100-TAK
Solid Phase Extraction (SPE) procedure:Note: The solvent volumes shown below are for a 30 mg bed mass. The solvent volumes will need to be adjusted for a smaller or larger bed mass.
Load:Load: 1 ml of analyte and internal standard (1.0 ug/ml) spiked porcine serum acidified with 2% conc.H3PO4 .
(Dilute the serum spiked analyte 1:1 with buffer or water as needed)
Dry:for 1 min at 10'' of Hg
Final Prep and Analysis: Blow down to almost dryness under slow stream of N2. Reconstitute with mobile phase to 200 uL Inject: 50 µL on HPLC / UV-Vis Abs.-Fixed Wave. (UV) @ 273 nm (ambient)

Analytes:

Name Spiked
Conc.
Log P pKa % Rec %RSC (n=0)
1 Theobromine 99
2 Theophylline 98
3 Beta hydroxy theophylline 97
4 Caffeine 96

Application Detail (App ID: 14449)

Part No:00F-4249-E0
Dimensions:150 x 4.6 mm ID
Elution Type:Isocratic
Elution A:20 mM KH2PO4, pH 2.5
Elution B:ACN
Gradient Profile:
Step No. Time (min) Pct A Pct B
1 0 95 5

Flow Rate:1.5 mL/min
Col. Temp: 25 °C
Detection: UV-Vis Abs.-Fixed Wave. (UV) @ 273 nm (22°C)
Note:SecurityGuard™ Guard Cartridge System extends column lifetime.
- SecurityGuard Cartridges, C8 4 x 3.0mm, 10/Pk Part No.: AJ0-4290
- Holder Part No.: KJ0-4282

Analyte Details

Analyte Detail not available

1
Theobromine
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2
Theophylline
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3
Beta hydroxy theophylline

4
Caffeine
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