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Extraction of Bronchodilators on Strata™ X

Sample Preparation Details

Condition 1 :1.0 mL Methanol
Condition 2 :1.0 mL Water
Load :Load 1.00 mL Sample diluted in Buffer
Wash 1 :1.0 mL 5% methanol/D. I. water
Wash 2 :1 at 2
Dry :for 1 min at 10'' of Hg
Elution 1 :1.0 mL Methanol
Evaporate: :Evaporate to dryness
Reconstitute :2 mL Mobile Phase
Notes :Blow down to almost dryness under slow stream of N2. Reconstitute with mobile phase to 200 uL

Application Detail (App ID: 14449)

Part No:00F-4249-E0
Dimensions:150 x 4.6 mm ID
Elution Type:Isocratic
Elution A:20 mM KH2PO4, pH 2.5
Elution B:ACN
Gradient Profile:
Step No. Time (min) Pct A Pct B
1 0 95 5

Flow Rate:1.5 mL/min
Col. Temp: 25 °C
Detection: UV-Vis Abs.-Fixed Wave. (UV) @ 273 nm (22°C)
Note:SecurityGuard™ Guard Cartridge System extends column lifetime.
- SecurityGuard Cartridges, C8 4 x 3.0mm, 10/Pk Part No.: AJ0-4290
- Holder Part No.: KJ0-4282

Analyte Details

Analyte Detail not available

1
Theobromine
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2
Theophylline
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3
Beta hydroxy theophylline

4
Caffeine
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