Extraction of Acetaminophen on Strata™ X

Sample Preparation Details

Part No:8B-S100-TAK
Solid Phase Extraction (SPE) procedure:Note: The solvent volumes shown below are for a 30 mg bed mass. The solvent volumes will need to be adjusted for a smaller or larger bed mass.
Load:Load: 1 ml of analyte and internal standard (1.0 ug/ml) spiked Porcine serum acidified with 2% conc.H3PO4 .
(Dilute the serum spiked analyte 1: 1 with buffer or water as needed)
Dry:For 1 min at 10'' of Hg
Final Prep and Analysis: Blow down to almost dryness under slow stream of N2.
Reconstitute with mobile phase to 200 uL.
Inject 50 uL on HPLC Inject: 50 µL on HPLC / UV-Vis Abs.-Fixed Wave. (UV) @ 220 nm (25 °C)


Name Spiked
Log P pKa % Rec %RSC (n=0)
1 Sulfanilimide
2 Acetaminophen 96 1.15

Application Detail (App ID: 14452)

Part No:00F-4249-E0
Dimensions:150 x 4.6 mm ID
Elution Type:Isocratic
Elution A:20 mM KH2PO4 (pH 7.0)
Elution B:Methanol
Gradient Profile:
Step No. Time (min) Pct A Pct B
1 0 90 10

Flow Rate:1 mL/min
Col. Temp: ambient
Detection: UV-Vis Abs.-Fixed Wave. (UV) @ 220 nm (25°C)
Note:SecurityGuard™ Guard Cartridge System extends column lifetime.
- SecurityGuard Cartridges, C8 4 x 3.0mm, 10/Pk Part No.: AJ0-4290
- Holder Part No.: KJ0-4282

Analyte Details

Analyte Detail not available