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Extraction of Fenoprofen on Strata™ X

Sample Preparation Details

Condition 1 : Methanol
Condition 2 : Water
Load :Load 1.00 mL Sample diluted in Buffer
Wash 1 :1.0 mL 5% methanol/D. I. water
Dry :1 min at 10" of Hg pressure
Elution 1 :1.0 mL Methanol
Elution 2 : Blow down to almost dryness under slow stream of N2.Reconstitute with mobile phase to 200 µL.
Evaporate: :Evaporate to dryness
Reconstitute :2 mL Buffer

Application Detail (App ID: 14455)

Part No:00F-4249-E0
Dimensions:150 x 4.6 mm ID
Elution Type:Isocratic
Elution A:0.05% H3PO4 (pH 2.3)
Elution B:ACN
Gradient Profile:
Step No. Time (min) Pct A Pct B
1 0 50 50

Flow Rate:1.5 mL/min
Col. Temp: ambient
Detection: UV-Vis Abs.-Fixed Wave. (UV) @ 230 nm (25°C)
Note:SecurityGuard™ Guard Cartridge System extends column lifetime.
- SecurityGuard Cartridges, C8 4 x 3.0mm, 10/Pk Part No.: AJ0-4290
- Holder Part No.: KJ0-4282

Analyte Details

Analyte Detail not available

1
Fenoprofen
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2
Indomethacine
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