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Applications
Water-Soluble Vitamins
14500
Separation Mode: Reversed Phase
Reversed Phase
C8(2)
HPLC
Vitamin B Complex
Pharmaceutical
Therapeutic / Clinical

Applications

Water-Soluble Vitamins
Analytes
1Thiamine
2Niacinamide
3Pyridoxine
4p-Aminobenzoic acid
5d-Pantothenic acid
6Folic acid
7Riboflavin
8d-Biotin
Compound Name
Thiamin
CID
1130
Molecular Formula
C12H17N4OS+
Molecular Weight
265
No. Hydrogen Bond Acceptors
4
No. Hydrogen Bond Donors
2
Total Formal Charge
1
Details
LC Conditions (App ID: 14500)
Column:
Brand Name:
Part No:
Phase Name:
C8(2)
Elution Type:
Gradient
Mobile Phase:
A: 0.1% H3PO4 in water
B: Acetonitrile/0.1% H3PO4 in water (80:20)
Gradient Profile:
Step No.Time(min)%A%B
10982
25982
3227228
4257228
Flow Rate:
0.8 mL/min
Temperature:
22°C
Detection:
UV-Vis Abs.-Variable Wave.(UV) @ 210 nm (22°C)
Sample Note:
SecurityGuard™ Guard Cartridge System extends column lifetime. - SecurityGuard Cartridges, C8 4 x 3.0mm, 10/Pk Part No.: AJ0-4290 - Holder Part No.: KJ0-4282

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