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Applications

Peptides from Serum on Strata™ X

Sample Preparation Details

Condition 1 :1.0 mL Methanol
Condition 2 :1.0 mL Water
Sample Pretreatment :Strata X 33u 30 mg / 1 ml Tube Condition: 1ml Methanol Load: 1 ml Spiked porcine serum extract
Load :Load 1.00 mL Sample
Wash 1 :1.0 mL 10% Acetonitrile in 0.1% TFA
Dry :1 min @ 10 in. Hg
Elution 1 :1.0 mL Acetonitrile:Water
Evaporate: :Evaporate to dryness
Reconstitute :300 µL TFA (0.1%) in Water
Notes :EVAPORATE AND RECONSTITUTE .40 °C under nitrogen stream 300 mL 0.1% aqueous TFA

Application Detail (App ID: 14616)

Part No:00F-4396-E0
Dimensions:150 x 4.6 mm ID
Elution Type:Gradient
Elution A:0.1% TFA
Elution B:ACN:0.1% TFA in water (90:10)
Gradient Profile:
Step No. Time (min) Pct A Pct B
1 0 85 15
2 22 40 60

Flow Rate:1 mL/min
Col. Temp: 30 °C
Detection: UV-Vis Abs.-Diode Array (PDA) @ 214 nm (22°C)
Note:SecurityGuard™ Guard Cartridge System extends column lifetime.
- SecurityGuard Cartridges, C12 4 x 3.0mm, 10/Pk Part No.: AJ0-6074
- Holder Part No.: KJ0-4282

Analyte Details

Analyte Detail not available

1
Oxytocin
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2
Angiotensin II
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3
Angiotensin I
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