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Applications

Peptides from Serum on Strata™ X

Sample Preparation Details

Part No:8B-S100-TAK
Solid Phase Extraction (SPE) procedure:Note: The solvent volumes shown below are for a 30 mg bed mass. The solvent volumes will need to be adjusted for a smaller or larger bed mass.
Load:Oxytocin, Angiotensin I,
Angiotensin II
5 ug/ml in 5mM KPhos pH2.5 Buffer
Dry:1 min @ 10 in. Hg
Final Prep and Analysis: EVAPORATE AND RECONSTITUTE .40 °C under nitrogen stream
300 mL 0.1% aqueous TFA Inject: 10 µL on HPLC / UV-Vis Abs.-Diode Array (PDA) @ 214 nm (ambient)

Analytes:

Name Spiked
Conc.
Log P pKa % Rec %RSC (n=0)
1 Oxytocin 99 99
2 Angiotensin II 96 96
3 Angiotensin I 96.5 96.5

Application Detail (App ID: 14616)

Part No:00F-4396-E0
Dimensions:150 x 4.6 mm ID
Elution Type:Gradient
Elution A:0.1% TFA
Elution B:ACN:0.1% TFA in water (90:10)
Gradient Profile:
Step No. Time (min) Pct A Pct B
1 0 85 15
2 22 40 60

Flow Rate:1 mL/min
Col. Temp: 30 °C
Detection: UV-Vis Abs.-Diode Array (PDA) @ 214 nm (22°C)
Note:SecurityGuard™ Guard Cartridge System extends column lifetime.
- SecurityGuard Cartridges, C12 4 x 3.0mm, 10/Pk Part No.: AJ0-6074
- Holder Part No.: KJ0-4282

Analyte Details

Analyte Detail not available

1
Oxytocin
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2
Angiotensin II
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3
Angiotensin I
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