Region

Applications

Steroids on Strata™ X

Sample Preparation Details

Condition 1 :1.0 mL Methanol
Condition 2 :1.0 mL Water
Load :Load 1.00 mL Sample
Wash 1 :1.0 mL Methanol:Water
Dry :1 min @ 10 in. Hg
Elution 1 :1.0 mL Methanol
Evaporate: :Evaporate to dryness
Reconstitute :200 µL Acetonitrile
Notes :Add 25 mL of 0.1 mg/ml butyl paraben (I.S.). 40 °C under N2 stream and reconstitute in 200 µL Acetonitrile

Application Detail (App ID: 14617)

Part No:00F-4337-E0
Dimensions:150 x 4.6 mm ID
Elution Type:Isocratic
Elution A:20 mM Potassium Phosphate, pH 7
Elution B:Methanol
Gradient Profile:
Step No. Time (min) Pct A Pct B
1 0 35 65

Flow Rate:1 mL/min
Col. Temp: ambient
Detection: UV-Vis Abs.-Variable Wave.(UV) @ 254 nm (22°C)
Note:SecurityGuard™ Guard Cartridge System extends column lifetime.
- SecurityGuard Cartridges, C12 4 x 3.0mm, 10/Pk Part No.: AJ0-6074
- Holder Part No.: KJ0-4282

Analyte Details

Analyte Detail not available

1
Prednisolone
search

2
Betamethasone
search

3
Butyl paraben
search

4
Betamethasone Valerate
search