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Applications

Steroids on Strata™ X

Sample Preparation Details

Part No:8B-S100-TAK
Solid Phase Extraction (SPE) procedure:Note: The solvent volumes shown below are for a 30 mg bed mass. The solvent volumes will need to be adjusted for a smaller or larger bed mass.
Load:1. Prednisolone (1ug/ml)
2. Betamethasone (1ug/ml)
3. Butyl paraben (I.S.) (0.1 mg/ml)
4. Betamethasone Valerate (4ug/ml)
Dry:1 min @ 10 in. Hg
Final Prep and Analysis: Add 25 mL of 0.1 mg/ml butyl paraben (I.S.). 40 °C under N2 stream and reconstitute in 200 µL Acetonitrile Inject: 5 µL on HPLC / UV-Vis Abs.-Variable Wave.(UV) @ 254 nm (ambient)

Analytes:

Name Spiked
Conc.
Log P pKa % Rec %RSC (n=0)
1 Prednisolone 94 3.7
2 Betamethasone 99 3.5
3 Butyl paraben 0.1 
4 Betamethasone Valerate 95 3.9

Application Detail (App ID: 14617)

Part No:00F-4337-E0
Dimensions:150 x 4.6 mm ID
Elution Type:Isocratic
Elution A:20 mM Potassium Phosphate, pH 7
Elution B:Methanol
Gradient Profile:
Step No. Time (min) Pct A Pct B
1 0 35 65

Flow Rate:1 mL/min
Col. Temp: ambient
Detection: UV-Vis Abs.-Variable Wave.(UV) @ 254 nm (22°C)
Note:SecurityGuard™ Guard Cartridge System extends column lifetime.
- SecurityGuard Cartridges, C12 4 x 3.0mm, 10/Pk Part No.: AJ0-6074
- Holder Part No.: KJ0-4282

Analyte Details

Analyte Detail not available

1
Prednisolone
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2
Betamethasone
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3
Butyl paraben
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4
Betamethasone Valerate
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