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Extraction of Diazepam on Strata™ X

Sample Preparation Details

Condition 1 :1.0 mL Methanol
Condition 2 :1.0 mL Water
Load :Load 1000.00 µL Sample
Wash 1 :1.0 mL Methanol:Water
Dry :1 min @ 10 in. Hg
Elution 1 : Methanol
Evaporate: :Evaporate to dryness
Reconstitute :200 µL Mobile Phase
Notes :Reconstitute with 200 µL mobile phase

Application Detail (App ID: 14618)

Part No:00F-4336-E0
Dimensions:150 x 4.6 mm ID
Elution Type:Gradient
Elution A:20 mM KH2PO4 (pH=2.5)
Elution B:MeOH
Gradient Profile:
Step No. Time (min) Pct A Pct B
1 0 75 25
2 3 75 25
3 8 30 70
4 11 30 70
5 14 75 25

Flow Rate:1.5 mL/min
Col. Temp: ambient
Detection: UV-Vis Abs.-Variable Wave.(UV) @ 254 nm (22°C)
Note:SecurityGuard™ Guard Cartridge System extends column lifetime.
- SecurityGuard Cartridges, POLAR-RP 4 x 3.0mm, 10/Pk Part No.: AJ0-6076
- Holder Part No.: KJ0-4282

Analyte Details

Analyte Detail not available

1
Desmethyldiazepam
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2
Diazepam
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