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Extraction of Barbiturates from Urine on Strata™ X

Sample Preparation Details

Part No:8B-S100-TAK
Solid Phase Extraction (SPE) procedure:Note: The solvent volumes shown below are for a 30 mg bed mass. The solvent volumes will need to be adjusted for a smaller or larger bed mass.
Load:Load: 1 ml of analyte & IS (conc. = 1.0 µg/mL) spiked Human urine (1:1 dilution with 100 mM KH2PO4, pH 6.0)
Final Prep and Analysis: Blow down under slow stream of N2 @ 40° C and reconstitute with 300 µL water Inject: 50 µL on HPLC / UV-Vis Abs.-Fixed Wave. (UV) @ 210 nm (ambient)

Analytes:

Name Spiked
Conc.
Log P pKa % Rec %RSC (n=0)
1 Phenobarbital 101
2 Butabarbital 103 0.46
3 Butalbital 100 0.33
4 Amobarbital 97 1.28
5 Secobarbital 90 2.79

Application Detail (App ID: 14622)

Part No:00F-4337-E0
Dimensions:150 x 4.6 mm ID
Elution Type:Gradient
Elution A:20 mM KH2PO4, pH 7.0
Elution B:Acetonitrile
Gradient Profile:
Step No. Time (min) Pct A Pct B
1 0 80 20
2 3 80 20
3 15 50 50
4 16 80 20

Flow Rate:1 mL/min
Col. Temp: ambient
Detection: UV-Vis Abs.-Fixed Wave. (UV) @ 210 nm (22°C)
Note:SecurityGuard™ Guard Cartridge System extends column lifetime.
- SecurityGuard Cartridges, C12 4 x 3.0mm, 10/Pk Part No.: AJ0-6074
- Holder Part No.: KJ0-4282

Analyte Details

Analyte Detail not available

1
Phenobarbital
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2
Butabarbital
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3
Butalbital
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4
Amobarbital
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5
Secobarbital
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