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Applications

Extraction of Barbiturates from Urine on Strata™ X

Sample Preparation Details

Condition 1 :1.0 mL Methanol
Condition 2 :1.0 mL Water
Load :Load 1.00 mL Sample diluted in KH2PO4 (100mM) pH 6.0
Wash 1 :1.0 mL Methanol / Water (5:95)
Wash 2 :1.0 mL Methanol / 2% CH3COOH in Water (10:90)
Elution 1 :1.0 mL Methanol / 2% Ammonium Hydroxide in Water (35:65)
Evaporate: :Evaporate to dryness
Reconstitute :300 µL Water
Notes :Blow down under slow stream of N2 @ 40° C and reconstitute with 300 µL water

Application Detail (App ID: 14622)

Part No:00F-4337-E0
Dimensions:150 x 4.6 mm ID
Elution Type:Gradient
Elution A:20 mM KH2PO4, pH 7.0
Elution B:Acetonitrile
Gradient Profile:
Step No. Time (min) Pct A Pct B
1 0 80 20
2 3 80 20
3 15 50 50
4 16 80 20

Flow Rate:1 mL/min
Col. Temp: ambient
Detection: UV-Vis Abs.-Fixed Wave. (UV) @ 210 nm (22°C)
Note:SecurityGuard™ Guard Cartridge System extends column lifetime.
- SecurityGuard Cartridges, C12 4 x 3.0mm, 10/Pk Part No.: AJ0-6074
- Holder Part No.: KJ0-4282

Analyte Details

Analyte Detail not available

1
Phenobarbital
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2
Butabarbital
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3
Butalbital
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4
Amobarbital
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5
Secobarbital
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