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Applications
Tryptic Digest of Cytochrome c on Luna SCX
14767
Separation Mode: Ion Exchange
Ion Exchange
SCX
HPLC
Peptide
Biochemical Compound / Nutrient

Applications

Tryptic Digest of Cytochrome c on Luna SCX
Analytes
1Cytochrome c
Compound Name
Myohematin
CID
439171
Molecular Formula
C42H52FeN8O6S2
Molecular Weight
884
No. Chiral Centers:
4
No. Hydrogen Bond Acceptors
12
No. Hydrogen Bond Donors
6
Details
LC Conditions (App ID: 14767)
Column:
Brand Name:
Part No:
Phase Name:
SCX
Elution Type:
Gradient
Mobile Phase:
A: 20mM KPhos pH 2.5 + 25% Acetonitrile
B: A + 350mM KCl
Gradient Profile:
Step No.Time(min)%A%B
111000
2500100
Flow Rate:
1 mL/min
Temperature:
30°C
Detection:
UV-Vis Abs.-Variable Wave.(UV) (22°C)
Sample Note:
SecurityGuard™ Guard Cartridge System extends column lifetime. - SecurityGuard Cartridges, SCX 4 x 3.0mm, 10/Pk Part No.: AJ0-4308 - Holder Part No.: KJ0-4282

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