Region

Applications

Simultaneous Desalting and Selective Elution of Basic, Acidic, and Neutral Peptides

Sample Preparation Details

Condition 1 :1.0mL Acetonitrile
Condition 2 :1.0mL Water
Load :Load 0.10 mL Sample diluted in Water
Wash 1 :1.0 mL Water
Elution 1 :0.25mL Acetonitrile:Water
Elution 2 :0.25mL Acetonitrile:Water
Elution 3 :0.25mL Acetonitrile:1% Formic Acid
Evaporate: :Evaporate to dryness
Reconstitute :0.60 mL Mobile phase

Application Detail (App ID: 14887)

Part No:00F-4337-E0
Dimensions:150 x 4.6 mm ID
Elution Type:Gradient
Elution A:0.1% TFA in Water
Elution B:0.1% TFA in Acetonitrile
Gradient Profile:
Step No. Time (min) Pct A Pct B
1 0 90 10
2 12 35 65

Flow Rate:1 mL/min
Col. Temp: ambient
Detection: UV-Vis Abs.-Variable Wave.(UV) @ 214 nm (22°C)
Note:SecurityGuard™ Guard Cartridge System extends column lifetime.
- SecurityGuard Cartridges, C12 4 x 3.0mm, 10/Pk Part No.: AJ0-6074
- Holder Part No.: KJ0-4282

Analyte Details

Analyte Detail not available

1
HGRH Grag 14-26

2
Angiotensin I
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3
Angiotensin II
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4
Angiotensin III
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5
Bradykinin
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