Region

Applications

Simultaneous Desalting and Selective Elution of Basic, Acidic, and Neutral Peptides

Sample Preparation Details

Part No:8B-S100-ECH
Solid Phase Extraction (SPE) procedure:Note: The solvent volumes shown below are for a 30 mg bed mass. The solvent volumes will need to be adjusted for a smaller or larger bed mass.
Final Prep and Analysis: Inject: 20 µL on HPLC / UV-Vis Abs.-Variable Wave.(UV) @ 214 nm (ambient)

Analytes:

Name Spiked
Conc.
Log P pKa % Rec %RSC (n=0)
1 HGRH Grag 14-26 102
2 Angiotensin I 100
3 Angiotensin II 106
4 Angiotensin III 104
5 Bradykinin 91

Application Detail (App ID: 14887)

Part No:00F-4337-E0
Dimensions:150 x 4.6 mm ID
Elution Type:Gradient
Elution A:0.1% TFA in Water
Elution B:0.1% TFA in Acetonitrile
Gradient Profile:
Step No. Time (min) Pct A Pct B
1 0 90 10
2 12 35 65

Flow Rate:1 mL/min
Col. Temp: ambient
Detection: UV-Vis Abs.-Variable Wave.(UV) @ 214 nm (22°C)
Note:SecurityGuard™ Guard Cartridge System extends column lifetime.
- SecurityGuard Cartridges, C12 4 x 3.0mm, 10/Pk Part No.: AJ0-6074
- Holder Part No.: KJ0-4282

Analyte Details

Analyte Detail not available

1
HGRH Grag 14-26

2
Angiotensin I
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3
Angiotensin II
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4
Angiotensin III
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5
Bradykinin
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