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Benzodiazepines (SPE-Plasma) on Hybrid C18 Column

Sample Preparation Details

Condition 1 :1mL Methanol
Condition 2 :1mL Water
Sample Pretreatment :SPE: Strata X 30mg/ml Sample: 0.5ml human plasam + 0.5ml water + 200ng benzodiazepines Condition: 1ml MeOH, 1ml Water Washing: 1ml water, 1ml 40%MeOH in water Elution: 1ml MeOH Reconstitution: 100ul water
Load :Load 1.00 mL Sample diluted in Water
Wash 1 :1mL Water
Wash 2 :1mL 40% Methanol in Water
Elution 1 :1mL Methanol
Evaporate: :Evaporate to dryness
Reconstitute :100 µL Water

Application Detail (App ID: 15454)

Part No:00F-4435-Y0
Dimensions:150 x 3 mm ID
Elution Type:Gradient
Elution A:0.1% Formic acid in Water
Elution B:0.1% Formic acid in Acetonitrile
Gradient Profile:
Step No. Time (min) Pct A Pct B
1 0 80 20
2 8 20 80

Flow Rate:0.8 mL/min
Col. Temp: ambient
Detection: UV-Vis Abs.-Variable Wave.(UV) @ 254 nm (22°C)
Note:SecurityGuard™ Guard Cartridge System extends column lifetime.
- SecurityGuard Cartridges, Gemini C18 4 x 2.0mm, 10/Pk Part No.: AJ0-7596
- Holder Part No.: KJ0-4282

Analyte Details

Analyte Detail not available

1
Chlodiazepoxide
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2
Flurazepam
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3
Oxazepam
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4
Clonazepam
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5
Temazepam
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6
Diazepam
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