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Benzodiazepines (SPE-Plasma) on Hybrid C18 Column

Sample Preparation Details

Part No:8B-S100-TAK
Solid Phase Extraction (SPE) procedure:Note: The solvent volumes shown below are for a 30 mg bed mass. The solvent volumes will need to be adjusted for a smaller or larger bed mass.
Load:0.5ml Human Plasma + 2ug/ml x 100ul + 0.5ml Water
(Spiked conc. in plasma:400ng/ml benzodiazepines)
Final Prep and Analysis: Inject: 40 µL on HPLC / UV-Vis Abs.-Variable Wave.(UV) @ 254 nm (ambient)

Analytes:

Name Spiked
Conc.
Log P pKa % Rec %RSC (n=0)
1 Chlodiazepoxide 200 
2 Flurazepam 200 
3 Oxazepam 200  82
4 Clonazepam 200 
5 Temazepam 200  96
6 Diazepam 200  98

Application Detail (App ID: 15454)

Part No:00F-4435-Y0
Dimensions:150 x 3 mm ID
Elution Type:Gradient
Elution A:0.1% Formic acid in Water
Elution B:0.1% Formic acid in Acetonitrile
Gradient Profile:
Step No. Time (min) Pct A Pct B
1 0 80 20
2 8 20 80

Flow Rate:0.8 mL/min
Col. Temp: ambient
Detection: UV-Vis Abs.-Variable Wave.(UV) @ 254 nm (22°C)
Note:SecurityGuard™ Guard Cartridge System extends column lifetime.
- SecurityGuard Cartridges, Gemini C18 4 x 2.0mm, 10/Pk Part No.: AJ0-7596
- Holder Part No.: KJ0-4282

Analyte Details

Analyte Detail not available

1
Chlodiazepoxide
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2
Flurazepam
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3
Oxazepam
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4
Clonazepam
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5
Temazepam
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6
Diazepam
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