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SCIEX 4000 QTRAP® LC/MS/MS system, Turbo V, source with TurboIonSpray® probe
TEM = 450°C, IS= 5000V, Declustering Potential (DP) = 30V, Collision Energy (CE) = optimized for each compound
Positive polarity, Multiple Reaction Monitoring of 297 transitions (MRMs of example see table)
Information Dependent Acquisition of 3 Enhanced Product Ion spectra to confirm detected compound at CE = 20, 35, and 50V, Dynamic fill time to avoid linear ion trap saturation, Dynamic background subtraction to allow detection of co-eluting peaks, Mass spectral library searching
Sample Note:
SecurityGuard™ Guard Cartridge System extends column lifetime.
- SecurityGuard Cartridges, Fusion-RP 4 x 2.0mm, 10/Pk Part No.: AJ0-7556
- Holder Part No.: KJ0-4282