| 1Pindolol |
|---|
| 2Metoprolol |
Compound Name Pindolol |
CID 4828 |
Molecular Formula C14H20N2O2 |
Molecular Weight 248 |
No. Chiral Centers: 1 |
No. Hydrogen Bond Acceptors 3 |
No. Hydrogen Bond Donors 3 |
Applications
Protein Precipatation vs. Ion Suppression of Medium Polar Drugs on strata Impact Square Well Plate
15712
Separation Mode: Reversed Phase
Reversed Phase
C18
HPLC
Antihypertensive Agents
Pharmaceutical
Therapeutic / Clinical
Applications
Protein Precipatation vs. Ion Suppression of Medium Polar Drugs on strata Impact Square Well Plate
Analytes
Details
LC Conditions (App ID: 15712)
Column: | ||||||||||||||||
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Phase Name: C18 | ||||||||||||||||
Elution Type: Gradient | ||||||||||||||||
Mobile Phase: A: 0.1% Formic acid/Water B: 0.08% Formic acid/Acetonitrile | ||||||||||||||||
Gradient Profile:
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Flow Rate: 0.5 mL/min | ||||||||||||||||
Temperature: 22°C | ||||||||||||||||
Detection: Mass Spectrometer (MS) @ 249 amu (22°C) | ||||||||||||||||
Sample Note: Protein Precipitation Protocol:
Phase: Strata Impact Square Well Plate, 2 mL (CEO-7565)
1. Dispense 300 uL acetonitrile into each well using an automatic pipettor.
2. Place the protein precipitation plate onto a suitable 96-well vacuum manifold . Make sure that a 96-well collection plate is positioned inside the manifold to collect the filtrate.
3. Dispense 100 uL of Porcine plasma into each well ( acetonitrile:plasma = 3:1). Let it stand for 2 mins (no vortex /mixing required).
4. Apply 5-10" of mercury for 30-40 secs.
5. Collect the filtrate and blow down to dryness under slow stream of nitrogen @ 40 deg. C.
6. Reconstitute with 100 uL of mobile phase containing 10.0 ng of analye.
Note: For ion suppression or enhancement estimation, a set of 4 blank (100 uL of water instead of plasma) was run in parallel.
Observation:
Filtrate looked very clean and clear
Results:
Analyte logP m/z % Variation Effect
1. Pindolol 1.75 249 15% Suppression
2. Metoprolol 1.88 268 13% Suppression |
Order Items Used in This Application
00F-4439-Y0
Gemini 3 µm C18 110 Å, LC Column 150 x 3 mm, Ea
Qty:
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