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20nt DNA Oligo-RP Purification - 20 Minute Dry Out

Sample Preparation Details

Condition 1 :1.5 mL Methanol/Water (1:1) at 1 mL/min
Condition 2 :6 mL Triophilic Buffer at 1 mL/min
Load :Load 1.50 mL Sample diluted in Triophilic Buffer
Wash 1 :8 mL Triophilic Buffer at 1 mL/min
Wash 2 :3 mL 2% TFA/50mM NaCl at 1 mL/min
Wash 3 :4 mL Water at 1 mL/min
Elution 1 :1 mL 40%Methanol/40%Water/20%Acetonitrile at 1 mL/min
Evaporate: :Evaporate to dryness

Application Detail (App ID: 15805)

Part No:00B-4441-E0
Dimensions:50 x 4.6 mm ID
Elution Type:Gradient
Elution A:50mM TEAA/ 5%ACN
Elution B:Methanol
Gradient Profile:
Step No. Time (min) Pct A Pct B
1 0 90 10
2 20 40 60

Flow Rate:1 mL/min
Col. Temp: ambient
Detection: UV-Vis Abs.-Variable Wave.(UV) @ 260 nm (22°C)
Note:Post conditioning 20 minute vacuum drying (compare with control sample AppID 15804)

Analyte Details

Analyte Detail not available

1
20 min Dry Out: DNA 20mer