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Applications
Basic Compounds at High pH: Gemini 10u C18 Axia Packed 50x21.2mm vs. Competitive 5u Column 50x19mm
15930
Separation Mode: Reversed Phase
Reversed Phase
C18
HPLC
Antidepressive Agents, Tricyclic
Organic Chemical
Chemical Compound

Applications

Basic Compounds at High pH: Gemini 10u C18 Axia Packed 50x21.2mm vs. Competitive 5u Column 50x19mm
Analytes
1Methacycline
2Triprolidine
3Amitriptyline
Compound Name
Methacycline
CID
5281054
Molecular Formula
C22H22N2O8
Molecular Weight
442
No. Hydrogen Bond Acceptors
10
No. Hydrogen Bond Donors
6
Details
LC Conditions (App ID: 15930)
Column:
Brand Name:
Part No:
Phase Name:
C18
Elution Type:
Gradient
Mobile Phase:
A: 95:5 50mM NH4HCO3 (pH 10.0) / Acetonitrile
B: 5:95 50mM NH4HCO3 (pH 10.0) / Acetonitrile
Gradient Profile:
Step No.Time(min)%A%B
101000
270100
Flow Rate:
30 mL/min
Temperature:
22°C
Detection:
UV-Vis Abs.-Variable Wave.(UV) @ 254 nm (22°C)
Sample Note:
Comparison of Gemini 10µm C18 Axia Packed column vs. leading 5µm competitive column. Flow rates adjusted to yield the same linear velocity. Contact your Technical Representative for more details.

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