Region

Applications

DNA / FAM-labeled DNA Cartridge Purification - Crude, Load, Wash, Elution Fractions

Sample Preparation Details

Condition 1 :1.5 mL 50% Methanol/Water at 1 mL/min
Condition 2 :6 mL Triophilic 3 in 1 Buffer at 1 mL/min
Load :Load 1.00 mL Sample diluted in Triophilic 3 in 1 Buffer
Wash 1 :8 mL Triophilic 3 in 1 Buffer at 1 mL/min
Elution 1 :1 mL Mehthanol/Water/Acetonitrile (40/40/20) at 1 mL/min
Evaporate: :Evaporate to dryness
Reconstitute :1 mL initial mobile phase

Application Detail (App ID: 15987)

Part No:00B-4441-E0
Dimensions:50 x 4.6 mm ID
Elution Type:Gradient
Elution A:50mM TEAA/5% Acetonitrile
Elution B:Methanol
Gradient Profile:
Step No. Time (min) Pct A Pct B
1 0 90 10
2 20 40 60

Flow Rate:
Col. Temp: ambient
Detection: UV-Vis Abs.-Variable Wave.(UV) @ 260 nm (22°C)
Note:SecurityGuard™ Guard Cartridge System extends column lifetime.
- SecurityGuard Cartridges, Clarity Oligo-RP 4 x 3.0mm, 10/Pk Part No.: AJ0-8135
- Holder Part No.: KJ0-4282

Analyte Details

Analyte Detail not available

1
DNA 20nt

2
FAM DNA 25nt