Region

Applications

Dye-labeled DNA Desalting - Crude, Load, Wash, Elution Fractions

Sample Preparation Details

Condition 1 :3 mL Methanol at 1 mL/min
Condition 2 :3 mL Water at 1 mL/min
Load :Load 2.00 mL Sample diluted in 0.2M TEAA
Wash 1 :3 mL 50mM TEAA/5% Acetonitrile at 1 mL/min
Elution 1 :1 mL Methanol/Water/Acetonitrile (40/40/20) at 1 mL/min
Evaporate: :Evaporate to dryness

Application Detail (App ID: 15991)

Part No:00B-4441-E0
Dimensions:50 x 4.6 mm ID
Elution Type:Gradient
Elution A:50mM TEAA/5% Acetonitrile
Elution B:Methanol
Gradient Profile:
Step No. Time (min) Pct A Pct B
1 0 90 10
2 20 40 60

Flow Rate:1 mL/min
Col. Temp: ambient
Detection: UV-Vis Abs.-Variable Wave.(UV) @ 260 nm (22°C)
Note:SecurityGuard™ Guard Cartridge System extends column lifetime.
- SecurityGuard Cartridges, Clarity Oligo-RP 4 x 3.0mm, 10/Pk Part No.: AJ0-8135
- Holder Part No.: KJ0-4282

Analyte Details

Analyte Detail not available

1
FAM Labeled DNA