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MercuryMS - Luna 3µm C18(2) 20 x 2.0mm for Benzodiazepines in Plasma by LC/MS/MS

Sample Preparation Details

Part No:8B-S100-TAK
Solid Phase Extraction (SPE) procedure:Note: The solvent volumes shown below are for a 30 mg bed mass. The solvent volumes will need to be adjusted for a smaller or larger bed mass.
Load:Spiked different concentration levels of benzodiazepines, metabolites and 50ng/mL clonazepam as IS into 200µL human plasma; diluted to 1mL with water
Final Prep and Analysis: Inject: 10 µL on HPLC / Mass Spectrometer (MS) @ amu (ambient)

Analytes:

Name Spiked
Conc.
Log P pKa % Rec %RSC (n=0)
1 7-Aminoflunitrazepam 100 
2 Chlordiazepoxide 100 
3 Oxazepam 100 
4 Nordazepam 100 
5 Clonazepam 50 
6 Temazepam 100 
7 Flunitrazepam 100 
8 Diazepam 100 

Application Detail (App ID: 16040)

Part No:00M-4251-B0-CE
Dimensions:20 x 2 mm ID
Elution Type:Gradient
Elution A:0.1% Formic Acid in Water
Elution B:0.1% Formic Acid in Acetonitrile
Gradient Profile:
Step No. Time (min) Pct A Pct B
1 0 85 15
2 1 5 95
3 1.01 85 15
4 2 85 15

Flow Rate:0.6 mL/min
Col. Temp: ambient
Detection: Mass Spectrometer (MS) @ amu (22°C)
Note:SecurityGuard™ Guard Cartridge System extends column lifetime.
- SecurityGuard Cartridges, C8 4 x 2.0mm, 10/Pk Part No.: AJ0-4289
- Holder Part No.: KJ0-4282

Analyte Details

Analyte Detail not available

1
7-Aminoflunitrazepam
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2
Chlordiazepoxide
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3
Oxazepam
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4
Nordazepam
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5
Clonazepam
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6
Temazepam
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7
Flunitrazepam
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8
Diazepam
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