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MercuryMS - Luna 3µm C18(2) 20 x 2.0mm for Benzodiazepines in Plasma by LC/MS/MS

Sample Preparation Details

Condition 1 :1 mL Methanol at 1 mL/min
Condition 2 :1 mL Water at 1 mL/min
Load :Load 1.00 mL Sample
Wash 1 :1 mL Water at 1.5 mL/min
Wash 2 :1 mL 20% MeOH in water at 1.5 mL/min
Elution 1 :1 mL Methanol at 0.5 mL/min
Evaporate: :Evaporate to dryness
Reconstitute :200 µL 15% CH3CN in water

Application Detail (App ID: 16040)

Part No:00M-4251-B0-CE
Dimensions:20 x 2 mm ID
Elution Type:Gradient
Elution A:0.1% Formic Acid in Water
Elution B:0.1% Formic Acid in Acetonitrile
Gradient Profile:
Step No. Time (min) Pct A Pct B
1 0 85 15
2 1 5 95
3 1.01 85 15
4 2 85 15

Flow Rate:0.6 mL/min
Col. Temp: ambient
Detection: Mass Spectrometer (MS) @ amu (22°C)
Note:SecurityGuard™ Guard Cartridge System extends column lifetime.
- SecurityGuard Cartridges, C8 4 x 2.0mm, 10/Pk Part No.: AJ0-4289
- Holder Part No.: KJ0-4282

Analyte Details

Analyte Detail not available

1
7-Aminoflunitrazepam
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2
Chlordiazepoxide
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3
Oxazepam
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4
Nordazepam
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5
Clonazepam
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6
Temazepam
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7
Flunitrazepam
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8
Diazepam
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