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MercuryMS - Synergi 2.5µm Fusion-RP 20 x 2.0mm for Tricyclics in Plasma by LC/MS/MS

Sample Preparation Details

Part No:8B-S029-TAK
Solid Phase Extraction (SPE) procedure:Note: The solvent volumes shown below are for a 30 mg bed mass. The solvent volumes will need to be adjusted for a smaller or larger bed mass.
Load:Spiked different concentration levels of tricyclic amines, its metabolites and 25ng/mL trimipramine as IS into 200µL human plasma; diluted to 1mL with water
Final Prep and Analysis: Inject: 4 µL on HPLC / Mass Spectrometer (MS) @ amu (ambient)

Analytes:

Name Spiked
Conc.
Log P pKa % Rec %RSC (n=0)
1 Doxepin 50 
2 Desipramine 50 
3 Imipramine 50 
4 Nortriptyline 50 
5 Trimipramine 25 
6 Amitriptyline 50 

Application Detail (App ID: 16041)

Part No:00M-4423-B0-CE
Dimensions:20 x 2 mm ID
Elution Type:Isocratic
Elution A:0.1% Formic Acid in Water
Elution B:0.1% Formic Acid in Acetonitrile
Gradient Profile:
Step No. Time (min) Pct A Pct B
1 0 28 72

Flow Rate:0.6 mL/min
Col. Temp: ambient
Detection: Mass Spectrometer (MS) @ amu (22°C)
Note:Total Ion Chromatogram is shown below. Single Ion Chromatograms are available. Please ask your Phenomenex Technical Representative.

Analyte Details

Analyte Detail not available

1
Doxepin
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2
Desipramine
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3
Imipramine
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4
Nortriptyline
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5
Trimipramine
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6
Amitriptyline
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