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MercuryMS - Luna 3µm C18(2) 20 x 2.0mm (mini column) for Tricyclics in Plasma by LC/MS/MS

Sample Preparation Details

Condition 1 :1 mL Methanol at 1 mL/min
Condition 2 :1 mL Water at 1 mL/min
Load :Load 1.00 mL Sample
Wash 1 :1 mL 0.1M acetic acid at 1.5 mL/min
Wash 2 :2 mL MeOH at 1.5 mL/min
Elution 1 :1 mL 5% ammonium hydroxide(28-30%) in MeOH at 0.5 mL/min
Evaporate: :Evaporate to dryness
Reconstitute :200 µL Mobile Phase

Application Detail (App ID: 16043)

Part No:00M-4251-B0
Dimensions:20 x 2 mm ID
Elution Type:Isocratic
Elution A:0.1% Formic Acid in Water
Elution B:0.1% Formic Acid in Acetonitrile
Gradient Profile:
Step No. Time (min) Pct A Pct B
1 0 28 72

Flow Rate:0.6 mL/min
Col. Temp: ambient
Detection: Mass Spectrometer (MS) @ amu (22°C)
Note:Total Ion Chromatogram is shown below. Single Ion Chromatograms are available. Please ask your Phenomenex Technical Representative.

Analyte Details

Analyte Detail not available

1
Doxepin
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2
Desipramine
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3
Imipramine
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4
Nortriptyline
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5
Trimipramine
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6
Amitriptyline
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