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Prep Scale Cartridge Purification of Phosphorothioate DNA

Sample Preparation Details

Condition 1 :10 mL Methanol/Water at 1 mL/min
Condition 2 :20 mL Triophilic Buffer at 1 mL/min
Load :Load 50.00 mL Sample diluted in 100mM TEAA
Wash 1 :40 mL 100mM TEAA/10% Acetonitrile/20% Methanol at 1 mL/min
Elution 1 : Methanol/Acetonitrile/Water
Evaporate: :Evaporate to dryness

Application Detail (App ID: 16068)

Part No:00B-4441-E0
Dimensions:50 x 4.6 mm ID
Elution Type:Gradient
Elution A:50M TEAA/5% Acetonitrile
Elution B:Methanol
Gradient Profile:
Step No. Time (min) Pct A Pct B
1 0 90 10
2 20 25 75

Flow Rate:1 mL/min
Col. Temp: ambient
Detection: UV-Vis Abs.-Variable Wave.(UV) @ 260 nm (22°C)
Note:SecurityGuard™ Guard Cartridge System extends column lifetime.
- SecurityGuard Cartridges, Clarity Oligo-RP 4 x 3.0mm, 10/Pk Part No.: AJ0-8135
- Holder Part No.: KJ0-4282

Analyte Details

Analyte Detail not available

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Phosphorothioate DNA