Region

Applications

Simultaneous Desalting and Selective Elution of Basic, Neutral and Acidic Peptides

Sample Preparation Details

Condition 1 :1 mL Acetonitrile
Condition 2 : Water
Load :Load 840.00 µL Sample diluted in water with salt
Wash 1 :2 mL Water
Elution 1 :0.25 mL 40:60 Acetonitrile/Water. for acidic peptides
Elution 2 :0.25 mL 70:30 Acetonitrile/Water. for neutral peptides
Elution 3 :0.25 mL 70:30 Acetonitrile/1.0% Formic Acid in Water. for basic peptides
Evaporate: :Evaporate to dryness
Reconstitute :20 µL initial mobile phase

Application Detail (App ID: 16087)

Part No:00F-4337-E0
Dimensions:150 x 4.6 mm ID
Elution Type:Gradient
Elution A:0.1% TFA in Water
Elution B:0.1% TFA in Acetonitrile
Gradient Profile:
Step No. Time (min) Pct A Pct B
1 0 90 10
2 12 35 65

Flow Rate:1 mL/min
Col. Temp: ambient
Detection: UV-Vis Abs.-Variable Wave.(UV) @ 214 nm (22°C)
Note:SecurityGuard™ Guard Cartridge System extends column lifetime.
- SecurityGuard Cartridges, C12 4 x 3.0mm, 10/Pk Part No.: AJ0-6074
- Holder Part No.: KJ0-4282

Analyte Details

Analyte Detail not available

1
Adrenocorticotropin Hormone Fragment 1-24 (ACTH)
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2
Bradykinin
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3
Methionine enkephalin
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4
Gonadotropin Releasing Hormone (HGRH) Fragment 14-26