Region

Applications

Simultaneous Desalting and Selective Elution of Basic, Neutral and Acidic Peptides

Sample Preparation Details

Part No:8B-S100-TAK
Solid Phase Extraction (SPE) procedure:Note: The solvent volumes shown below are for a 30 mg bed mass. The solvent volumes will need to be adjusted for a smaller or larger bed mass.
Load:500ul of sodium chloride (0.1M) + 100ul ACTH (250ug/ml) +100ul
Bradykinin (250ug.ml) + 100ul HGRH (250ug/ml) + 40ul Met-Enkephalin (250ug/ml)
Final Prep and Analysis: Inject: 20 µL on HPLC / UV-Vis Abs.-Variable Wave.(UV) @ 214 nm (ambient)

Analytes:

Name Spiked
Conc.
Log P pKa % Rec %RSC (n=0)
1 Adrenocorticotropin Hormone Fragment 1-24 (ACTH) 70  79
2 Bradykinin 70  92.5
3 Methionine enkephalin 30  100
4 Gonadotropin Releasing Hormone (HGRH) Fragment 14-26 102

Application Detail (App ID: 16087)

Part No:00F-4337-E0
Dimensions:150 x 4.6 mm ID
Elution Type:Gradient
Elution A:0.1% TFA in Water
Elution B:0.1% TFA in Acetonitrile
Gradient Profile:
Step No. Time (min) Pct A Pct B
1 0 90 10
2 12 35 65

Flow Rate:1 mL/min
Col. Temp: ambient
Detection: UV-Vis Abs.-Variable Wave.(UV) @ 214 nm (22°C)
Note:SecurityGuard™ Guard Cartridge System extends column lifetime.
- SecurityGuard Cartridges, C12 4 x 3.0mm, 10/Pk Part No.: AJ0-6074
- Holder Part No.: KJ0-4282

Analyte Details

Analyte Detail not available

1
Adrenocorticotropin Hormone Fragment 1-24 (ACTH)
search

2
Bradykinin
search

3
Methionine enkephalin
search

4
Gonadotropin Releasing Hormone (HGRH) Fragment 14-26