Overlay of PEGylated vs. Native Proteins on Jupiter® 300 C18 Column

Application Detail (App ID: 16192)

Part No:00F-4053-E0
Dimensions:150 x 4.6 mm ID
Elution Type:Gradient
Elution A:0.1% TFA and 2% ACN in Water
Elution B:70/20% ACN/IPA, 0.08% TFA in Water
Gradient Profile:
Step No. Time (min) Pct A Pct B
1 0 85 15
2 25 30 70

Flow Rate:1 mL/min
Col. Temp: 45 °C
Detection: UV-Vis Abs.-Variable Wave.(UV) @ 214 nm (22°C)
Note:Reversed phase of PEGylated and native proteins run on a Jupiter 300 C4 column. Note the decrease in resolution of PEGylated form versus the C4 column.

Analyte Details

Analyte Detail not available

PEGylated vs. Native Proteins