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Applications

PEGylated Insulin on Jupiter® 300 C4, C18 and Two Experimental Columns

Application Detail (App ID: 16193)

Part No:00F-4167-E0
Dimensions:150 x 4.6 mm ID
Elution Type:Gradient
Elution A:0.1% TFA and 2% ACN in Water
Elution B:70/20% ACN/IPA, 0.08% TFA in Water
Gradient Profile:
Step No. Time (min) Pct A Pct B
1 0 85 15
2 25 30 70

Flow Rate:1 mL/min
Col. Temp: 45 °C
Detection: UV-Vis Abs.-Variable Wave.(UV) @ 214 nm (22°C)
Note:Reversed phase of PEGylated Insulin run on listed columns. Note the reduced resolution for the Jupiter C18 and EXP 2 columns when compared to the Jupiter C4 column. Although it is difficult to visualize, the Jupiter actully has better resolution of PEGylated forms than the EXP1 column.

Analyte Details

Analyte Detail not available

1
PEGylated Insulin