Region

Applications

Jupiter® 300 C4 - Comparison of Mobile Phase Conditions

Application Detail (App ID: 16194)

Part No:00F-4167-E0
Dimensions:150 x 4.6 mm ID
Elution Type:Gradient
Elution A:0.1% TFA in Water
Elution B:90% ACN 0.09% TFA in Water
Gradient Profile:
Step No. Time (min) Pct A Pct B
1 0 80 20
2 25 45 55

Flow Rate:1 mL/min
Col. Temp: 45 °C
Detection: UV-Vis Abs.-Variable Wave.(UV) @ 214 nm (22°C)
Note:Reversed phase runs of PEGylated carbonic anhydrase and PEGylated ribonuclease A using different organic mobile phase compositions. Unlike other application, the addition of isopropanol does not improve resolution of different PEGylated forms.

Analyte Details

Analyte Detail not available

1
PEGylated Carbonic anhydrase or PEGylated Ribonucluease A