Jupiter® 300 C4 - Comparison of Mobile Phase Conditions

Application Detail (App ID: 16194)

Part No:00F-4167-E0
Dimensions:150 x 4.6 mm ID
Elution Type:Gradient
Elution A:0.1% TFA in Water
Elution B:90% ACN 0.09% TFA in Water
Gradient Profile:
Step No. Time (min) Pct A Pct B
1 0 80 20
2 25 45 55

Flow Rate:1 mL/min
Col. Temp: 45 °C
Detection: UV-Vis Abs.-Variable Wave.(UV) @ 214 nm (22°C)
Note:Reversed phase runs of PEGylated carbonic anhydrase and PEGylated ribonuclease A using different organic mobile phase compositions. Unlike other application, the addition of isopropanol does not improve resolution of different PEGylated forms.

Analyte Details

Analyte Detail not available

PEGylated Carbonic anhydrase or PEGylated Ribonucluease A