Jupiter® 300 C4 - Comparison of Tetrahydrofuran Organic Mixtures

Application Detail (App ID: 16195)

Part No:00F-4167-E0
Dimensions:150 x 4.6 mm ID
Elution Type:Gradient
Elution A:0.1% TFA in Water
Elution B:90% ACN 0.09% TFA in Water
Gradient Profile:
Step No. Time (min) Pct A Pct B
1 0 80 20
2 25 45 55

Flow Rate:1 mL/min
Col. Temp: 45 °C
Detection: UV-Vis Abs.-Variable Wave.(UV) @ 214 nm (22°C)
Note:Reversed phase runs of PEGylated beta lactoglobin using different organic mobile phase compositions on Jupiter 300 C4. 70% acetonitrile/ 20% tetrahydrofuran was substuted for the organic portion of the mobile phase. For various proteins tested the addition of THF slightly decreased the resolution of PEGylated forms.

Analyte Details

Analyte Detail not available

PEGylated beta-Lactoglobulin