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SPE of Tetracyclines from Honey Using Gemini 5u

Sample Preparation Details

Condition 1 :2 mL MeOH at 1 mL/min
Condition 2 :2 mL Water at 1 mL/min
Sample Pretreatment :The SPE extract looks so clean that conc. down to 50 ng/mL (100ng on column injection by conentrating the SPE extract) can be achievable without much background noise.
Load :Load 4.00 mL Sample diluted in NaOAc (100mM) pH 5.0
Wash 1 :4 mL Water at 1 mL/min
Wash 2 :4 mL Methanol at 1 mL/min
Wash 3 :2 mL 2% Formic Acid in Methanol at 1 mL/min
Dry :2 min at 10" of Hg
Elution 1 :2 mL 2% Formic Acid in Methanol at 1 mL/min
Evaporate: :Evaporate to dryness
Reconstitute :100 µL 10mM oxalic acid (pH2)/ACN=80/20
Notes :Elution of 2 mL (2% Formic Acid in Methanol) is recommended to do in 2 aliquots

Application Detail (App ID: 16318)

Part No:00F-4435-Y0
Dimensions:150 x 3 mm ID
Elution Type:Isocratic
Elution A:10 mm Oxalic acid
Elution B:Acetonitrile
Gradient Profile:
Step No. Time (min) Pct A Pct B
1 0 80 20

Flow Rate:0.8 mL/min
Col. Temp: ambient
Detection: UV-Vis Abs.-Variable Wave.(UV) @ 365 nm (22°C)
Note:SecurityGuard™ Guard Cartridge System extends column lifetime.
- SecurityGuard Cartridges, Gemini C18 4 x 2.0mm, 10/Pk Part No.: AJ0-7596
- Holder Part No.: KJ0-4282

Analyte Details

Analyte Detail not available

1
Oxytetracycline
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2
Tetracycline
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3
Chlortetracycline
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