| 1Nornicotine |
|---|
| 2Nicotine |
| 3Cotinine |
Compound Name Nornicotine |
CID 412 |
Molecular Formula C9H12N2 |
Molecular Weight 148 |
No. Chiral Centers: 1 |
No. Hydrogen Bond Acceptors 2 |
No. Hydrogen Bond Donors 1 |
Applications
Nicotine and Metabolites on Gemini 5µm C18
16345
Separation Mode: Reversed Phase
Reversed Phase
C18
HPLC
Indicators and Reagents
Clinical
Pharmaceutical
Therapeutic / Clinical
Analytes
Details
LC Conditions (App ID: 16345)
Column: | ||||||||||||||||||||||||
Brand Name: | ||||||||||||||||||||||||
Part No: | ||||||||||||||||||||||||
Phase Name: C18 | ||||||||||||||||||||||||
Elution Type: Gradient | ||||||||||||||||||||||||
Mobile Phase: A: Water with 0.1% v/v Formic Acid B: Acetonitrile with 0.1% v/v Formic Acid | ||||||||||||||||||||||||
Gradient Profile:
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Flow Rate: 0.4 mL/min | ||||||||||||||||||||||||
Temperature: 22°C | ||||||||||||||||||||||||
Detection: Mass Spectrometer (MS) (22°C) | ||||||||||||||||||||||||
Detector Info: SCIEX API 3000™ Triple Quadrupole Mass Spectrometer with TurboIonSpray Source
MRM transitions monitored:
Nicotine: m/z 163.3/105.9 and 163.3/80.1
Nornicotine: m/z 149.1/80.1
Cotinine: m/z 177.3/98.0 and 177.3/80.1 | ||||||||||||||||||||||||
Sample Note: The pH of the aqueous acid is 2.7. 0.5ng on-column injection of each analyte. |
Order Items Used in This Application
00D-4435-B0
Gemini 5 µm C18 110 Å, LC Column 100 x 2 mm, Ea
Qty:
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Hydrophilic Interaction
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