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Nicotine and Metabolites on Gemini 5µm C18

Application Detail (App ID: 16345)

Part No:00D-4435-B0
Dimensions:100 x 2 mm ID
Elution Type:Gradient
Elution A:Water with 0.1% v/v Formic Acid
Elution B:Acetonitrile with 0.1% v/v Formic Acid
Gradient Profile:
Step No. Time (min) Pct A Pct B
1 0 97 3
2 1 97 3
3 3 70 30
4 3.01 97 3
5 5.5 97 3

Flow Rate:0.4 mL/min
Col. Temp: ambient
Detection: Mass Spectrometer (MS) @ amu (22°C)
Detector Info: AB Sciex API 3000™ Triple Quadrupole Mass Spectrometer with TurboIonSpray Source
MRM transitions monitored:
Nicotine: m/z 163.3/105.9 and 163.3/80.1
Nornicotine: m/z 149.1/80.1
Cotinine: m/z 177.3/98.0 and 177.3/80.1
Note:The pH of the aqueous acid is 2.7. 0.5ng on-column injection of each analyte.

Analyte Details

Analyte Detail not available

1
Nornicotine
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2
Nicotine
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3
Cotinine
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