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Sterols from Lard on ZB-5MS

Sample Preparation Details

Condition 1 :5 mL Methanol
Condition 2 :5 mL Chloroform
Load :Load 0.70 mL Sample diluted in 20 ml 0.5 M KOH in Ethanol
Elution 1 : 5 % Methanol in Chloroform
Evaporate: :Evaporate to dryness
Notes :Initial Sample Unit: gram

Application Detail (App ID: 16514)

Part No:7HG-G010-11
Phase:5% Phenyl-Arylene 95% Dimethylpolysiloxane
Dimensions:30 meters x 0.25 mm x 0.25 µm
Oven Profile:225 °C to 330 °C @ 15 °C/min for 2 min.
Carrier Gas:Constant Flow Helium, 2.5 mL/min
Injection:Splitless :1 0.5 µL @ 320°C
Detection: Flame Ionization (FID) (320°C)
Note:Sample prep- Saponification, Solid Phase Extraction (SPE), and Derivatization.

Analyte Details

Analyte Detail not available

1
Cholesterol
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2
Betulin
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