PEGylated proteins on Jupiter® 300 C18 3µm and 5µm, and C4 5µm columns

Application Detail (App ID: 17521)

Part No:00F-4263-E0
Dimensions:150 x 4.6 mm ID
Elution Type:Gradient
Elution A:0.1% TFA, 2% ACN in Water
Elution B:90% ACN, 0.085% TFA in Water
Gradient Profile:
Step No. Time (min) Pct A Pct B
1 0 80 20
2 25 35 65

Flow Rate:1 mL/min
Col. Temp: 45 °C
Detection: UV-Vis Abs.-Variable Wave.(UV) @ 214 nm (25°C)
Note:Compare reverse phase separations of different PEGylated and intact proteins selected at different reaction time-points on three different columns. (A) PEGylated alpha-Chymotrypsinogen A with PEG1 Amine 20kDa Methoxy PEG NHS-ester, 0 min fraction; (B) PEGylated alpha-Chymotrypsinogen A with PEG3 N-Terminus 20kDa Methoxy PEG aldehyde, 120 min fraction; (C) PEGylated beta-Lactoglobulin A with PEG4 N-Terminus 40kDa Y-shape Methoxy PEG aldehyde, overnight fraction. Well-noticed, good resolution was achieved on Jupiter 3µm C18 small particles versus larger Jupiter 5µm C18 particles or Jupiter C4 bonded media.

Analyte Details

Analyte Detail not available

PEGylated Proteins