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THC and Metabolite in Plasma by LC/MS/MS on Gemini C18 5µm 50 x 3.0

Sample Preparation Details

Condition 1 :1 mL Methanol
Condition 2 :1 mL DI Water
Load :Load 2.00 mL Sample diluted in 1mL Plasma diluted 1:1 with Water
Wash 1 :1 mL 0.1N HAC
Wash 2 :1 mL 30% ACN in 0.1N HAC
Wash 3 :0.2
Elution 1 :1 mL 2% HAC in CH3CN by gravity
Evaporate: :Evaporate to dryness
Reconstitute :100 µL 15:85/ACN:10mM NH4HCO3, pH=10.0

Application Detail (App ID: 17901)

Part No:00B-4439-Y0
Dimensions:50 x 3 mm ID
Elution Type:Gradient
Elution A:10mM NH4HCO3 (pH 10)
Elution B:Acetonitrile
Gradient Profile:
Step No. Time (min) Pct A Pct B
1 0.1 85 15
2 3 5 95
3 3.5 5 95
4 3.6 85 15
5 4.1 85 15

Flow Rate:0.8 mL/min
Col. Temp: ambient
Detection: Mass Spectrometer (MS) @ amu (450°C)
Note:SecurityGuard™ Guard Cartridge System extends column lifetime.
- SecurityGuard Cartridges, Gemini C18 4 x 2.0mm, 10/Pk Part No.: AJ0-7596
- Holder Part No.: KJ0-4282

Analyte Details

Analyte Detail not available

1
11-nor-9-Carboxy-THC (THC-COOH)
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2
delta-9-tetrahydrocannabinol-d3 (THC)
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3
delta-9-tetrahydrocannabinol (THC)
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