Region

Applications

THC and Metabolite in Plasma by LC/MS/MS on Gemini C18 5µm 50 x 3.0

Sample Preparation Details

Part No:8B-S029-TAK
Solid Phase Extraction (SPE) procedure:Note: The solvent volumes shown below are for a 30 mg bed mass. The solvent volumes will need to be adjusted for a smaller or larger bed mass.
Load:100ul x 100ng/ml(in MeOH, evaporated) std + 1ml human plasma + 1ml Water (conc. spiked in plasma: 10ng/ml)
Final Prep and Analysis: Inject: 20 µL on HPLC / Mass Spectrometer (MS) @ amu (450 °C)

Analytes:

Name Spiked
Conc.
Log P pKa % Rec %RSC (n=0)
1 11-nor-9-Carboxy-THC (THC-COOH)
2 delta-9-tetrahydrocannabinol-d3 (THC)
3 delta-9-tetrahydrocannabinol (THC)

Application Detail (App ID: 17901)

Part No:00B-4439-Y0
Dimensions:50 x 3 mm ID
Elution Type:Gradient
Elution A:10mM NH4HCO3 (pH 10)
Elution B:Acetonitrile
Gradient Profile:
Step No. Time (min) Pct A Pct B
1 0.1 85 15
2 3 5 95
3 3.5 5 95
4 3.6 85 15
5 4.1 85 15

Flow Rate:0.8 mL/min
Col. Temp: ambient
Detection: Mass Spectrometer (MS) @ amu (450°C)
Note:SecurityGuard™ Guard Cartridge System extends column lifetime.
- SecurityGuard Cartridges, Gemini C18 4 x 2.0mm, 10/Pk Part No.: AJ0-7596
- Holder Part No.: KJ0-4282

Analyte Details

Analyte Detail not available

1
11-nor-9-Carboxy-THC (THC-COOH)
search

2
delta-9-tetrahydrocannabinol-d3 (THC)
search

3
delta-9-tetrahydrocannabinol (THC)
search