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Applications

LC/MS Separation poly dT 12-18 (Figure 3)

Analytes

1poly dT (12-18) ladder

Compound Name Thymidylic Acid
CID 9700
Molecular Formula C10H15N2O8P
Molecular Weight 322
No. Hydrogen Bond Acceptors 8
No. Hydrogen Bond Donors 4

Details

LC Conditions (App ID: 17966)

Column:

Clarity 3 µm Oligo-RP, LC Column 100 x 2 mm, Ea

Brand Name:

Part No:

Phase Name:

Oligo-RP

Elution Type:

Gradient

Mobile Phase:

A: 100mM HFIP/4mMTEA/2% MeOH

B: 100mM HFIP/4mMTEA/2% MeOH

Gradient Profile:

Step No.	Time(min)	%A	%B
1	0.01	95	5
2	1	95	5
3	5	50	50
4	5.5	40	60
5	6.5	20	80
6	7	95	5
7	10	95	5

Flow Rate:

0.2 mL/min

Temperature:

50°C

Detection:

Mass Spectrometer (MS) (400°C)

Sample Note:

SecurityGuard™ Guard Cartridge System extends column lifetime. - SecurityGuard Cartridges, Clarity Oligo-RP 4 x 2.0mm, 10/Pk Part No.: AJ0-8134 - Holder Part No.: KJ0-4282

Order Items Used in This Application

00D-4441-B0

Clarity 3 µm Oligo-RP, LC Column 100 x 2 mm, Ea

Qty:

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