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Applications

Plasma Metanephrines by LC/MS/MS on Luna HILIC following SPE with Strata-X-CW

Sample Preparation Details

Condition 1 :400 µL Methanol/Acetonitrile (50:50)
Condition 2 :400 µL Water
Load :Load 1.50 mL Sample diluted in 500 uL Plasma diluted with 1 mL Water
Wash 1 :800 µL Water
Wash 2 :2000 µL Methanol/Acetonitrile (50:50)
Dry :2 min at 10" Hg
Elution 1 :200 µL 5% Formic acid (freshly prepared) in 50/50 Acetonitrile/Methanol
Elution 2 :200 µL 5% Formic acid (freshly prepared) in 50/50 Acetonitrile/Methanol
Evaporate: :Evaporate to dryness
Reconstitute :100 µL 95/5 Acetonitrile/100 mM Ammonium formate, pH 3.2
Notes :Elution solvent must be freshly prepared

Application Detail (App ID: 17970)

Part No:00B-4449-B0
Dimensions:50 x 2 mm ID
Elution Type:Gradient
Elution A:ACN/100mM Ammonium Formate, pH 3.2 (95:5)
Elution B:ACN/Water/100mM Ammonium Formate, pH 3.2 (50:45:5)
Gradient Profile:
Step No. Time (min) Pct A Pct B
1 0 100 0
2 2.51 0 100
3 3.01 100 0
4 6 100 0

Flow Rate:0.4 mL/min
Col. Temp: ambient
Detection: Mass Spectrometer (MS) @ amu (22°C)
Detector Info: Analyte MRM Pair
(Q1/Q3)
MN 180.4/148.2
MN-D3 183.4/151.2
NMN 166.4/134.2
NMN-D3 169.4/137.2
Note:SecurityGuard™ Guard Cartridge System extends column lifetime.
- SecurityGuard Cartridges, HILIC 4 x 2.0mm, 10/Pk Part No.: AJ0-8328
- Holder Part No.: KJ0-4282

Analyte Details

Analyte Detail not available

1
Metanephrine
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2
Metanephrine-d3
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3
Normetanephrine
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4
Normetanephrine-d3
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