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Steroids in Standard Urine on Gemini 5µm C18 150 x 3mm ID

Sample Preparation Details

Part No:8B-S100-TAK
Solid Phase Extraction (SPE) procedure:Note: The solvent volumes shown below are for a 30 mg bed mass. The solvent volumes will need to be adjusted for a smaller or larger bed mass.
Load:Spiked human serum or urine (diluted 1:1 with water)
Final Prep and Analysis: Inject: 10 µL on HPLC / Mass Spectrometer (MS) @ amu (450 °C)

Analytes:

Name Spiked
Conc.
Log P pKa % Rec %RSC (n=0)
1 Triamcinolone
2 Prednisolone
3 Cortisone
4 Betamethasone
5 Corticosterone
6 Triamcinolone acetonide
7 11-alpha-Hydroxyprogesterone
8 Cortisone acetate
9 11-Ketoprogesterone
10 Betamethasone 17-valerate

Application Detail (App ID: 17971)

Part No:00F-4435-Y0
Dimensions:150 x 3 mm ID
Elution Type:Gradient
Elution A:0.1 % Formic Acid in Water
Elution B:0.1 % Formic Acid in 95% Acetonitrile/Water
Gradient Profile:
Step No. Time (min) Pct A Pct B
1 0 60 40
2 4 100 0
3 15 25 75
4 15.1 40 60
5 20 40 60

Flow Rate:0.6 mL/min
Col. Temp: ambient
Detection: Mass Spectrometer (MS) @ amu (450°C)
Note:SecurityGuard™ Guard Cartridge System extends column lifetime.
- SecurityGuard Cartridges, Gemini C18 4 x 2.0mm, 10/Pk Part No.: AJ0-7596
- Holder Part No.: KJ0-4282

Analyte Details

Analyte Detail not available

1
Triamcinolone
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2
Prednisolone
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3
Cortisone
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4
Betamethasone
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5
Corticosterone
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6
Triamcinolone acetonide
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7
11-alpha-Hydroxyprogesterone
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8
Cortisone acetate
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9
11-Ketoprogesterone
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10
Betamethasone 17-valerate
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