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Steroids in a Blank Urine Sample on Gemini 5µm C18 150 x 3mm ID

Sample Preparation Details

Condition 1 :1 mL Methanol
Condition 2 :1 mL Water
Load :Load 1.00 mL Sample diluted in Human serum or urine
Wash 1 :1 mL Water
Wash 2 :1 mL 40% Methanol in Water
Elution 1 :1 mL Methanol
Evaporate: :Evaporate to dryness
Reconstitute :100 µL 100 uL acetonitrile/water 40:60

Application Detail (App ID: 17972)

Part No:00F-4435-Y0
Dimensions:150 x 3 mm ID
Elution Type:Gradient
Elution A:0.1 % Formic Acid in Water
Elution B:0.1 % Formic Acid in 95% Acetonitrile/Water
Gradient Profile:
Step No. Time (min) Pct A Pct B
1 0 60 40
2 4 100 0
3 15 25 75
4 15.1 40 60
5 20 40 60

Flow Rate:0.6 mL/min
Col. Temp: ambient
Detection: Mass Spectrometer (MS) @ amu (450°C)
Note:SecurityGuard™ Guard Cartridge System extends column lifetime.
- SecurityGuard Cartridges, Gemini C18 4 x 2.0mm, 10/Pk Part No.: AJ0-7596
- Holder Part No.: KJ0-4282

Analyte Details

Analyte Detail not available

1
Triamcinolone
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2
Prednisolone
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3
Cortisone
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4
Betamethasone
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5
Corticosterone
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6
Triamcinolone acetonide
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7
11-alpha-Hydroxyprogesterone
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8
Cortisone acetate
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9
11-Ketoprogesterone
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10
Betamethasone 17-valerate
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