| 1Poly dT 12-18 Oligonucleotide |
|---|
Compound Name Thymidylic Acid |
CID 9700 |
Molecular Formula C10H15N2O8P |
Molecular Weight 322 |
No. Hydrogen Bond Acceptors 8 |
No. Hydrogen Bond Donors 4 |
Applications
Poly dT 12-18 standard on Oligo-RP (modifying TEA/HFIP Ratio)
18089
Separation Mode: Reversed Phase
Reversed Phase
Oligo-RP
HPLC
Life Science
Pharmaceutical/Biopharmaceutical
Oligonucleotide
Biochemical Compound / Nutrient
Analytes
Details
LC Conditions (App ID: 18089)
Column: | ||||||||||||||||
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Phase Name: Oligo-RP | ||||||||||||||||
Elution Type: Gradient | ||||||||||||||||
Mobile Phase: A: TEAA/HFIP/2%MeOH in Water B: Acetonitrile | ||||||||||||||||
Gradient Profile:
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Flow Rate: 0.2 mL/min | ||||||||||||||||
Temperature: 22°C | ||||||||||||||||
Detection: Mass Spectrometer (MS) @ 400 amu (22°C) | ||||||||||||||||
Sample Note: Different ratios of HFIP to TEA were investigated using the poly dT 12-18 standard mixture. HFIP was fixed at 200 mM and different levels of TEA were used between 1 and 20 mM (4mm-red trace {pH 7.6}, 8mm-blue trace{pH8.0} and 14mm -black trace{pH8.3}). Optimal retention, MS response, and resolution were observed for the 200 mM HFIP./ 8mM TEA mobile phase mixture. |
Order Items Used in This Application
00F-4441-B0
Clarity 3 µm Oligo-RP, LC Column 150 x 2 mm, Ea
Qty:
Similar Applications
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Poly dT 12-18 Oligonucleotide
Clarity 2.6 µm Oligo-MS 100A, LC Column 50 x 2.1 mm, Ea
Reversed Phase
Clarity 2.6 µm Oligo-MS 100A, LC Column 50 x 2.1 mm, Ea
Reversed Phase
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