1Poly dT 12-18 Oligonucleotide |
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Compound Name Thymidylic Acid |
CID 9700 |
Molecular Formula C10H15N2O8P |
Molecular Weight 322 |
No. Hydrogen Bond Acceptors 8 |
No. Hydrogen Bond Donors 4 |
1Poly dT 12-18 Oligonucleotide |
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Compound Name Thymidylic Acid |
CID 9700 |
Molecular Formula C10H15N2O8P |
Molecular Weight 322 |
No. Hydrogen Bond Acceptors 8 |
No. Hydrogen Bond Donors 4 |
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Phase Name: Oligo-RP | ||||||||||||||||
Elution Type: Gradient | ||||||||||||||||
Mobile Phase: A: TEAA/HFIP/2%MeOH in Water B: Acetonitrile | ||||||||||||||||
Gradient Profile:
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Flow Rate: 0.2 mL/min | ||||||||||||||||
Temperature: 22°C | ||||||||||||||||
Detection: Mass Spectrometer (MS) @ 400 amu (22°C) | ||||||||||||||||
Sample Note: Different ratios of HFIP to TEA were investigated using the poly dT 12-18 standard mixture. HFIP was fixed at 200 mM and different levels of TEA were used between 1 and 20 mM (4mm-red trace {pH 7.6}, 8mm-blue trace{pH8.0} and 14mm -black trace{pH8.3}). Optimal retention, MS response, and resolution were observed for the 200 mM HFIP./ 8mM TEA mobile phase mixture. |