Poly dT 12-18 standard on Oligo-RP (modifying TEA/HFIP Ratio)

Application Detail (App ID: 18089)

Part No:00F-4441-B0
Dimensions:150 x 2 mm ID
Elution Type:Gradient
Elution A:TEAA/HFIP/2%MeOH in Water
Elution B:Acetonitrile
Gradient Profile:
Step No. Time (min) Pct A Pct B
1 0 100 0
2 10 90 10
3 30 70 30

Flow Rate:0.2 mL/min
Col. Temp: ambient
Detection: Mass Spectrometer (MS) @ 400 amu (22°C)
Note:Different ratios of HFIP to TEA were investigated using the poly dT 12-18 standard mixture. HFIP was fixed at 200 mM and different levels of TEA were used between 1 and 20 mM (4mm-red trace {pH 7.6}, 8mm-blue trace{pH8.0} and 14mm -black trace{pH8.3}). Optimal retention, MS response, and resolution were observed for the 200 mM HFIP./ 8mM TEA mobile phase mixture.

Analyte Details

Analyte Detail not available

Poly dT 12-18 Oligonucleotide