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Simultaneous Extraction of Melamine and Cyanuric acid from Strata Melamine on Luna HILIC

Sample Preparation Details

Condition 1 :3 mL Methanol at 1.0 mL/min
Condition 2 :3 mL Acetonitrile:Water (50/50) at 1.0 mL/min
Load :Load 3.50 mL Sample diluted in Acetonitrile:Water
Wash 1 :0.5 mL Acetonitrile:Water (50/50); 2 aliquots of at 1.0 mL/min
Wash 2 :0.5 mL Methanol:Water (50/50); 1 aliquot of at 1.0 mL/min
Wash 3 :2 at 1.0
Dry :2 mins at 10" of Hg
Elution 1 :0.5 mL Methanol at 1.0 mL/min
Elution 2 :0.5 mL 5% Ammonium Hydroxide in Methanol; 2 aliquots of at 1.0 mL/min
Evaporate: :Evaporate to dryness
Reconstitute :1 mL 90/10 : ACN/100mM ammonium formate, pH 3.2

Application Detail (App ID: 18287)

Part No:00D-4449-B0
Dimensions:100 x 2 mm ID
Elution Type:Isocratic
Elution A:90/10 ACN/100 mM Ammonium Formate, pH 3.2
Gradient Profile:
Step No. Time (min) Pct A
1 3.5 100

Flow Rate:0.4 mL/min
Col. Temp: ambient
Detection: Mass Spectrometer (MS) @ (22°C)
Detector Info: * MS in -ve ionization till 1.7 mins then to +ve till 3.5 mins
Note:SecurityGuard™ Guard Cartridge System extends column lifetime.
- SecurityGuard Cartridges, HILIC 4 x 2.0mm, 10/Pk Part No.: AJ0-8328
- Holder Part No.: KJ0-4282

Analyte Details

Analyte Detail not available

1
Cyanuric acid
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2
Melamine
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