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Extraction of Sulfa Drugs from Porcine Plasma using Strata X-C

Sample Preparation Details

Part No:8B-S029-TAK-S
Solid Phase Extraction (SPE) procedure:Note: The solvent volumes shown below are for a 30 mg bed mass. The solvent volumes will need to be adjusted for a smaller or larger bed mass.
Load:Load 0.5 mL of spiked plasma , diluted with 1 mL of water (acidified with 2% phosphoric acid)
Dry:1 min at 10" of Hg
Final Prep and Analysis: Inject: 20 µL on HPLC / UV-Vis Abs.-Variable Wave.(UV) @ 254 nm (ambient)

Analytes:

Name Spiked
Conc.
Log P pKa % Rec %RSC (n=0)
1 Sulfanilamide 0.5 
2 Sulfathiazole 0.5  99
3 Sulfamethoxazole 0.5  89
4 Sulfaquinoxaline 0.5  88

Application Detail (App ID: 18293)

Part No:00F-4337-E0
Dimensions:150 x 4.6 mm ID
Elution Type:Gradient
Elution A:20 mm KH2PO4 (pH2.5)
Elution B:ACN
Gradient Profile:
Step No. Time (min) Pct A Pct B
1 0 95 5
2 15 20 80

Flow Rate:1 mL/min
Col. Temp: ambient
Detection: UV-Vis Abs.-Variable Wave.(UV) @ 254 nm (22°C)
Note:SecurityGuard™ Guard Cartridge System extends column lifetime.
- SecurityGuard Cartridges, C12 4 x 3.0mm, 10/Pk Part No.: AJ0-6074
- Holder Part No.: KJ0-4282

Analyte Details

Analyte Detail not available

1
Sulfanilamide
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2
Sulfathiazole
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3
Sulfamethoxazole
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4
Sulfaquinoxaline
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