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Extraction of Sulfa Drugs from Porcine Plasma using Strata X-C

Sample Preparation Details

Condition 1 :1 mL Methanol at 1 mL/min
Condition 2 :1 mL Water at 1 mL/min
Load :Load 1.50 mL Sample diluted in Water
Wash 1 :1 mL HCl (0.1N) at 1 mL/min
Wash 2 :1 mL Methanol at 1 mL/min
Dry :1 min at 10" of Hg
Elution 1 :1 mL 5% Ammonium Hydroxide in Methanol at 1 mL/min
Evaporate: :Evaporate to dryness
Reconstitute :200 µL KH2PO4 (20mM) pH 2.5

Application Detail (App ID: 18293)

Part No:00F-4337-E0
Dimensions:150 x 4.6 mm ID
Elution Type:Gradient
Elution A:20 mm KH2PO4 (pH2.5)
Elution B:ACN
Gradient Profile:
Step No. Time (min) Pct A Pct B
1 0 95 5
2 15 20 80

Flow Rate:1 mL/min
Col. Temp: ambient
Detection: UV-Vis Abs.-Variable Wave.(UV) @ 254 nm (22°C)
Note:SecurityGuard™ Guard Cartridge System extends column lifetime.
- SecurityGuard Cartridges, C12 4 x 3.0mm, 10/Pk Part No.: AJ0-6074
- Holder Part No.: KJ0-4282

Analyte Details

Analyte Detail not available

1
Sulfanilamide
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2
Sulfathiazole
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3
Sulfamethoxazole
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4
Sulfaquinoxaline
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