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Benzoylecgonine from urine by Strata-XC on a ZB-Drug1

Sample Preparation Details

Part No:8B-S029-TBJ
Solid Phase Extraction (SPE) procedure:Note: The solvent volumes shown below are for a 30 mg bed mass. The solvent volumes will need to be adjusted for a smaller or larger bed mass.
Load:To 1mL of urine add 1mL of 100mM phosphate buffer (pH 6.0), adjust pH to 6.0 using 100 mM monobasic or dibasic sodium phosphate.
Dry:5 minutes at > 10 inch Hg
Final Prep and Analysis: Inject: 1 µL on GC / Mass Selective (MSD) @ amu / (320 °C)

Analytes:

Name Spiked
Conc.
Log P pKa % Rec %RSC (n=0)
1 Benzoylecgonine-d3
2 Benzoylecgonine

Application Detail (App ID: 18416)

Part No:7CD-G023-08
Phase:Proprietary Drug Phase
Dimensions:10 meters x 0.18 mm x 0.18 µm
Oven Profile:235 °C to 320 °C @ 25 °C/min for 1min
Carrier Gas:Constant Flow Helium, 55 cm/sec
Injection:Split 10:1 1 µL @ 260°C
Detection: Mass Selective (MSD) (320°C)
Note:Analytes are 300ng/mL extracted from urine then derivatized with BSTFA
Sim ions:
(Quant ion*) D3-Benzoylecgonine (*85,243,364): Benzoylecgonine (*82,240,361)

Analyte Details

Analyte Detail not available

1
Benzoylecgonine-d3
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2
Benzoylecgonine
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