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Extraction of Sulfonamides from Honey by LC/MS/MS Using strata X-C and Gemini 5u, C18, 150x3.0

Sample Preparation Details

Condition 1 :2 mL Methanol at 1 mL/min
Condition 2 :2 mL Water at 1 mL/min
Load :Load 1.00 mL Sample diluted in 1mL of 2M HCl and 3mL 0.3M Citric acid
Wash 1 :4 mL Water (2 aliquots of 2 mL) at 1 mL/min
Wash 2 :4 mL MeOH/ACN (50/50) (2 aliquots of 2 mL) at 1 mL/min
Dry :2 mins at 10" of Hg pressure
Elution 1 :2 mL 2% ammonium hydroxide/MeOH at 1 mL/min
Evaporate: :Evaporate to dryness
Reconstitute :0 µL Mobile Phase
Notes :Addition of IS followed by evaporation before reconstitution: Add IS (Sulfaquinoxaline) and evaporate the sample under slow stream of nitrogen @ 40 deg C and reconstitute with 100 uL of mobile phase

Application Detail (App ID: 18458)

Part No:00F-4435-Y0
Dimensions:150 x 3 mm ID
Elution Type:Gradient
Elution A:0.1% Formic acid/water
Elution B:0.1% formic acid/ACN
Gradient Profile:
Step No. Time (min) Pct A Pct B
1 0 90 10
2 8 30 70

Flow Rate:0.6 mL/min
Col. Temp: ambient
Detection: Mass Spectrometer (MS) @ amu (22°C)
Note:HPLC system: HP 1100 MS detector: API 3000, LC/MS/MS,Turbo spray: ESI, positive ion mode; MRM; heater gas flow 7000 cc/min; heater temp: 425° C

Analyte Details

Analyte Detail not available

1
Sulfanilamide
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2
Sulfathiazole
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3
Sulfamerazine
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4
Sulfamethoxazole
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5
Sulfaquinoxaline
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