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Carboxy-THC from urine by Strata-XC on a ZB-Drug1

Sample Preparation Details

Condition 1 :1 mL Methanol at 1 mL/min
Condition 2 :1 mL Water at 1 mL/min
Condition 3 :1 at 1
Load :Load 2.00 mL Sample diluted in Urine
Wash 1 :1 mL 0.1 N HCl at 1 mL/min
Wash 2 :1 mL 0.1 N HCl/ACN (70:30) at 1 mL/min
Dry :5 minutes at > 10 inch Hg
Elution 1 :1 mL 2% Acetic acid in acetonitrile at 1 mL/min
Evaporate: :Evaporate to dryness
Reconstitute :50 µL BSTFA
Notes :After drying the cartridge (before elution step) add 200 µL hexane and aspirate to remove any residual moisture.

Application Detail (App ID: 18481)

Part No:7CD-G023-08
Phase:Proprietary Drug Phase
Dimensions:10 meters x 0.18 mm x 0.18 µm
Oven Profile:220 °C to 320 °C @ 20 °C/min for 5 min
Carrier Gas:Constant Flow Helium, 0.7 mL/min
Injection:Split 10:1 1 µL @ 260°C
Detection: Mass Selective (MSD) (320°C)
Note:Analytes are 15ng/mL in urine derivatized with BSTFA
Sim ions:
(Quant ion*) D3-Carboxy-thc (*374,476,477): Carboxy-thc (*371,473,474)

Analyte Details

Analyte Detail not available

1
Carboxy-THC-d3

2
Carboxy-THC