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Methadone from urine by Strata-XC on a ZB-Drug1

Sample Preparation Details

Part No:8B-S029-TBJ
Solid Phase Extraction (SPE) procedure:Note: The solvent volumes shown below are for a 30 mg bed mass. The solvent volumes will need to be adjusted for a smaller or larger bed mass.
Load:To 0.5mL of urine add 1mL of 100mM phosphate buffer (pH 6.0), adjust pH to 6.0 using phosphoric acid
Dry:5 minutes at > 10 inch Hg
Final Prep and Analysis: Inject: 1 µL on GC / Mass Selective (MSD) @ amu / (320 °C)

Analytes:

Name Spiked
Conc.
Log P pKa % Rec %RSC (n=0)
1 Methadone-d9
2 Methadone

Application Detail (App ID: 18485)

Part No:7CD-G023-08
Phase:Proprietary Drug Phase
Dimensions:10 meters x 0.18 mm x 0.18 µm
Oven Profile:180 °C to 340 °C @ 25 °C/min for 3.6 min
Carrier Gas:Constant Flow Helium, 55 cm/sec
Injection:Split 10:1 1 µL @ 280°C
Detection: Mass Selective (MSD) (320°C)
Note:Methadone/Methadone-d9 is 300/600ng/mL respectively in urine
Sim ions:
(Quant ion*) D9-Methadone (*78,303): Methadone (*72,294,223)

Analyte Details

Analyte Detail not available

1
Methadone-d9
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2
Methadone
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