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Methadone from urine by Strata-XC on a ZB-Drug1

Sample Preparation Details

Condition 1 :1 mL Methanol at 1 mL/min
Condition 2 :1 mL Water at 1 mL/min
Condition 3 :2 at 1
Load :Load 1.00 mL Sample diluted in Urine
Wash 1 :1 mL 0.1 N HCl at 1 mL/min
Wash 2 :1 mL Methanol at 1 mL/min
Wash 3 :3 at 1
Dry :5 minutes at > 10 inch Hg
Elution 1 : 5% Ammonium hydroxide in methanol
Evaporate: :Evaporate to dryness
Reconstitute :50 µL Ethyl acetate

Application Detail (App ID: 18485)

Part No:7CD-G023-08
Phase:Proprietary Drug Phase
Dimensions:10 meters x 0.18 mm x 0.18 µm
Oven Profile:180 °C to 340 °C @ 25 °C/min for 3.6 min
Carrier Gas:Constant Flow Helium, 55 cm/sec
Injection:Split 10:1 1 µL @ 280°C
Detection: Mass Selective (MSD) (320°C)
Note:Methadone/Methadone-d9 is 300/600ng/mL respectively in urine
Sim ions:
(Quant ion*) D9-Methadone (*78,303): Methadone (*72,294,223)

Analyte Details

Analyte Detail not available

1
Methadone-d9
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2
Methadone
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