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6-MAM from urine by Strata-XC on a ZB-Drug1

Sample Preparation Details

Condition 1 :1 mL Methanol at 1 mL/min
Condition 2 :1 mL Water at 1 mL/min
Condition 3 :1 at 1
Load :Load 1.00 mL Sample diluted in Urine
Wash 1 :1 mL 0.1 N HCl at 1 mL/min
Wash 2 :1 mL Methanol at 1 mL/min
Wash 3 :3 at 1
Dry :10minutes at > 10 inch Hg
Elution 1 :1 mL Ammonium hydroxide in methanol 5% at 1 mL/min
Evaporate: :Evaporate to dryness
Reconstitute :50 µL BSTFA/Pyridine

Application Detail (App ID: 18487)

Part No:7CD-G023-08
Phase:Proprietary Drug Phase
Dimensions:10 meters x 0.18 mm x 0.18 µm
Oven Profile:150 °C to 300 °C @ 30 °C/min
Carrier Gas:Constant Flow Helium, 0.3 mL/min
Injection:Split 10:1 1 µL @ 260°C
Detection: Mass Selective (MSD) (320°C)
Note:Analytes are 10ng/mL in urine derivatized with BSTFA
Sim ions:
(Quant ion*) D3-6-MAM (*402,403): 6-MAM (*399,400,340)

Note: Method kept this way for continuity, the sample can be cleaned furthur by using different wash steps. For more information contact a Phenomenex technical consultant.

Analyte Details

Analyte Detail not available

1
6-MAM-d3
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2
6-MAM
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