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6-MAM from urine by Strata-XC on a ZB-Drug1

Sample Preparation Details

Part No:8B-S029-TAK
Solid Phase Extraction (SPE) procedure:Note: The solvent volumes shown below are for a 30 mg bed mass. The solvent volumes will need to be adjusted for a smaller or larger bed mass.
Load:To 1mL of urine add 1mL of 100mM phosphate buffer (pH 6.0), adjust pH to 6.0 using phosphoric acid
Dry:10minutes at > 10 inch Hg
Final Prep and Analysis: Inject: 1 µL on GC / Mass Selective (MSD) @ amu / (320 °C)

Analytes:

Name Spiked
Conc.
Log P pKa % Rec %RSC (n=0)
1 6-MAM-d3
2 6-MAM

Application Detail (App ID: 18487)

Part No:7CD-G023-08
Phase:Proprietary Drug Phase
Dimensions:10 meters x 0.18 mm x 0.18 µm
Oven Profile:150 °C to 300 °C @ 30 °C/min
Carrier Gas:Constant Flow Helium, 0.3 mL/min
Injection:Split 10:1 1 µL @ 260°C
Detection: Mass Selective (MSD) (320°C)
Note:Analytes are 10ng/mL in urine derivatized with BSTFA
Sim ions:
(Quant ion*) D3-6-MAM (*402,403): 6-MAM (*399,400,340)

Note: Method kept this way for continuity, the sample can be cleaned furthur by using different wash steps. For more information contact a Phenomenex technical consultant.

Analyte Details

Analyte Detail not available

1
6-MAM-d3
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2
6-MAM
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