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Atenolol-Related Substance (European Pharmacopoeia) on Kinetex 2.6µm C18 100 x 4.6mm ID

Application Detail (App ID: 18519)

Part No:00D-4462-E0
Dimensions:100 x 4.6 mm ID
Elution Type:Isocratic
Elution A:12.5 mM KH2PO4 in Water, pH 3.0 + 2.0g Sodium Octanesulfonate + 0.8g Tetrabutyl Ammonium Hydrogen Sulfate
Elution B:Methanol
Elution C:THF
Gradient Profile:
Step No. Time (min) Pct A Pct B Pct C
1 0 80 18 2

Flow Rate:1 mL/min
Col. Temp: ambient
Detection: UV-Vis Abs.-Variable Wave.(UV) @ 226 nm (22°C)
Note:Atenolol mobile phase was prepared as follows:
2.0 g of sodium octanesulphonate + 0.8 g of tetrabutylammonium hydrogen sulphate in 2 L of 360ml methanol + 40 mL of THF + 1600 mL of 3.4 g of potassium dihydrogen phosphate in 2 L of water (pH adjusted to 3.0- phosphoric acid)

Analyte Details

Analyte Detail not available

1
Impurity B

2
Impurity A

3
Impurity J
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4
Impurity I
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5
Impurity D and E

6
Impurity F

7
Impurity G

8
Impurity H