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Applications
6-MAM on Kinetex 2.6µm C18 50 x 2.1mm ID with Acquity UPLC MS/MS
18607
Separation Mode: Reversed Phase
Reversed Phase
C18
HPLC
Toxicology / Drug Screening / TDM
Therapeutic / Clinical

Applications

6-MAM on Kinetex 2.6µm C18 50 x 2.1mm ID with Acquity UPLC MS/MS
Analytes
16-MAM
26-MAM-d3
Compound Name
6-Acetylmorphine
CID
5462507
Molecular Formula
C19H21NO4
Molecular Weight
327
No. Hydrogen Bond Acceptors
5
No. Hydrogen Bond Donors
1
Details
LC Conditions (App ID: 18607)
Column:
Brand Name:
Kinetex
Part No:
Phase Name:
C18
Elution Type:
Gradient
Mobile Phase:
A: 0.1% Formic acid in H2O
B: 0.1% Formic acid in ACN
Gradient Profile:
Step No.Time(min)%A%B
109010
213565
31.01595
41.5595
51.519010
639010
Flow Rate:
700 µL/min
Temperature:
0°C
Detection:
Mass Spectrometer (MS) (110°C)
Detector Info:
ESI+, MRM mode 6-monoacetylmorphine (6-MAM) - 328.3-152.3 (quantitation) & 328.3-165.3 (qualifier) d3-6-MAM - 331.3-211.3 (internal standard)

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