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Extraction of Carbamate Pesticides using Strata-X on Kinetex 2.6µm C18 50 x 2.1mm ID by LCMS

Sample Preparation Details

Part No:8B-S100-TBJ
Solid Phase Extraction (SPE) procedure:Note: The solvent volumes shown below are for a 30 mg bed mass. The solvent volumes will need to be adjusted for a smaller or larger bed mass.
Load:Load 500 uL apple juice (spiked with 50uL of analyte and 50 uL of IS) dilute with 1 mL of buffer (25 mM ammonium acetate pH 5.5)
Dry:2 mins at 10" of Hg
Final Prep and Analysis: Inject: 5 µL on HPLC / Mass Spectrometer (MS) @ amu (ambient)

Analytes:

Name Spiked
Conc.
Log P pKa % Rec %RSC (n=0)
1 Aldicarb-sulfoxide 200 
2 Aldicarb-sulfone 200 
3 Oxamyl 200 
4 Methomyl 200 
5 3-Hydroxy carbofuran 200 
6 Aldicarb 200 
7 Carbofuran and D3 200 
8 Methiocarb 200 

Application Detail (App ID: 18638)

Part No:00B-4462-AN
Dimensions:50 x 2.1 mm ID
Elution Type:Gradient
Elution A:5mM Ammonium formate in H2O
Elution B:mM Ammonium formate in ACN
Gradient Profile:
Step No. Time (min) Pct A Pct B
1 0 97 3
2 0.25 97 3
3 2.5 5 95
4 4 5 95
5 4.01 97 3
6 5.5 97 3

Flow Rate:500 µL/min
Col. Temp: 25 °C
Detection: Mass Spectrometer (MS) @ amu (22°C)
Note:SecurityGuard™ ULTRA Guard Cartridge System extends column lifetime.
- SecurityGuard ULTRA Cartridges, UHPLC C18 for 2.1mm ID Columns, 3/Pk Part No.: AJ0-8782
- Holder Part No.: AJ0-9000

Analyte Details

Analyte Detail not available

1
Aldicarb-sulfoxide
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2
Aldicarb-sulfone
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3
Oxamyl
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4
Methomyl
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5
3-Hydroxy carbofuran
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6
Aldicarb
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7
Carbofuran and D3
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8
Methiocarb
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